Protein Purification MCQs 0.0 / 5 ? OtherBiochemistryUniversityNone Created by: undeadsmurf2014Created on: 14-12-21 02:50 Amino acids have a positive and negative charge. Molecules with this characteristic are known as? Zwitterionic 1 of 27 What character of the peptide bond provides stability to peptide chains? Partial double bond character 2 of 27 B-pleated sheets in a protein structure are stabilised by? Inter chain H-bonds 3 of 27 The shape a protein finally assumes is driven by? Accommodating internal hydrophobic amino acid side chains 4 of 27 Discrete areas in a protein structure which convey functionality are known as Motifs and Domains 5 of 27 The isoelectric point of an amino acid or protein is given by the symbol? pl 6 of 27 Electrophoresis is incomplete electrolysis. What determines the overall movement velocity of a particle subjected to an electrical field? All of the answers 7 of 27 Non-denaturing polyacrylamide gel electrophoresis (native PAGE) separates protein according to their ... Charge and mass 8 of 27 Electrophoresis in the presence of a detergent (SDS-PAGE) separates polypeptides by ... their size 9 of 27 Samples for electrophoresis in the presence of a detergent (SDS-PAGE) are boiled with 2-mercaptoethanol to break? Covalent disulphide bonds 10 of 27 In a proteins' secondary structure, an alpha helix is... a right-handed helix 11 of 27 There are? amino acids in each turn of the alpha helix 3.6 12 of 27 Beta strands in a proteins structure are stabilised by inter chain H-bonds 13 of 27 What is the approximate time for a haemoglobin alpha subunit (Mr 16,000) to be synthesised? 90 sec 14 of 27 Polypeptides are more than 10 amino acids linked together 15 of 27 What is the minimum pH that needs to be used to make a protein with a pI of 6.5 move to the anode in non-denaturing PAGE 6.75-7.0 16 of 27 The movement of proteins in SDS-PAGE is related to... The log10 of their molecular weight 17 of 27 In isoelectric focusing proteins will Move depending on their charge 18 of 27 In a protein's secondary structure an alpha helix is... a right-handed helix 19 of 27 Proteins that share the same domain have identical function 20 of 27 After electrophoresis proteins can be stained with ... All answers 21 of 27 In 2D electrophoresis what electrophoretic techniques are used and in what order? IEF followed by SDS-PAGE 22 of 27 What technique can be used to determine the molecular weight of a protein sample? SDS-PAGE, Size-exclusion chromatography and Mass spectroscopy 23 of 27 The area of Bioinformatics combines computing and biology 24 of 27 What techniques can be used to sequence a protein? Mass spectrometry and Edman degradation 25 of 27 What types of detectors are used in mass spectroscopy? Time of flight 26 of 27 What enzyme is used to degrade a protein sample prior to mass spec analysis? Trypsin 27 of 27
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