Microbial Growth Pt1

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What are the factors affecting microbial growth?
Temperature
pH
Aeration
Moisture content
Presence of inhibitory substances
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What type of microorganisms can grow at -5-20 degrees
Psychrophiles
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What type of microorganisms can grow at 15-45 degrees
Mesophiles
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What type of microorganisms can grow at 42-80 degrees
Thermophiles
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What type of microorganisms can grow at 75-105 degrees
Hyperthermophiles
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What type of microorganisms can grow at pH 1-4
Acidophile
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What type of microorganisms can grow at pH 8-12
Alkaliphile
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What group of microorganisms tend to be more acid-tolerant
Fungi
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What type of microorganisms only grow in the presence of oxygen
Obligate aerobes
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What type of microorganisms only grow in the absence of oxygen
Obligate anaerobes
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What do obligate anaerobes use as a substitute for oxygen?
Nitrogen
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What type of microorganisms grows in the presence of oxygen but can switch their metabolic pathway and grow if there is an absence of oxygen?
Facultative anaerobes
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What type of microorganisms can grow at any concentration of oxygen
Aerotolerant anaerobes
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Why is CO2 an important factor?
pH changes
Inhibitory at high concentrations
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What does water availability depend on?
Absolute water content of the environment
Concentration of solutes e.g. salt
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What is water availability expressed as?
Water activity (aw)
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What is water activity?
The relationship between the moisture content of the substrate and the relative humidity of the air surrounding it
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What are the survival structures in fungi?
Spores
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What are the survival structures in bacteria?
Spores and cysts
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What are the population growth phases?
Lag phase
Exponential phase
Stationary Phase
Death phase
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What is the phase that lies between the exponential and stationary phase called?
Deceleration phase
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What forms during the deceleration phase?
Survival structures
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Why do fungi produce spores?
Survival
Dispersal
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What are the two main types of spore?
Sexual
Asexual
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What are sexual spores?
Thick-walled
Resistant to unfavourable environmental conditions
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What are asexual spores?
Produced in large numbers for dispersal
Low resistance to unfavourable conditions
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What are the two genera of Gram-positive bacteria that can form endospores inside vegetative cells?
Bacillus
Clostridium
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What is a vegetative cell?
A growing cell
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What do endospores do?
Withstand extreme conditions of desiccation
Remain viable in a metabolically inactive state
And then germinate when conditions become favourable
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What compound do endospores have large amounts of?
Calcium dipicolinate
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What is a bacterial cyst?
Modified vegetative cell
Develops a thick chemically and physically resistant cell wall
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Card 2

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What type of microorganisms can grow at -5-20 degrees

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Psychrophiles

Card 3

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What type of microorganisms can grow at 15-45 degrees

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Card 4

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What type of microorganisms can grow at 42-80 degrees

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Card 5

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What type of microorganisms can grow at 75-105 degrees

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