gene technology 4.5 / 5 based on 4 ratings ? BiologyPractical applications of biologyA2/A-levelAQA Created by: EmmaCreated on: 03-04-13 15:29 restriction endonuclease DNA 'scissors', cuts at specific palindromic sequences 1 of 10 reverse transcriptase conversion of mRNA to cDNA 2 of 10 DNA ligase joins the sugar-phosphate backbone 3 of 10 'in vivo' cloning 'in cell', vectors used to insert a gene into host cells 4 of 10 'in vitro' cloning 'out of cell', PCR clones directly 5 of 10 DNA gel electrophoresis used to seperate pieces of DNA (based on length), can locate a specific fragment, DNA contaions a (-) phosphate group, so moves towards (+) 6 of 10 a vector is a carrier into a host cell, often use plasmids 7 of 10 a plasmid a small circular piece of DNA, can replicate indpendently, can move between bacteria easily (vertical/horizontal gene transmission) 8 of 10 importance of using the same restriction enzyme DNA and vector will have comlementary 'sticky ends', and so will pair up 9 of 10 recombinant DNA DNA fragment + plasmid 10 of 10
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