Biology-B3-Monoclonal Antibodies

?
  • Created by: tonisha_
  • Created on: 26-06-22 18:52
Antibodies are produced by what?

Monoclonal antibodies are produced from a single?
lymphocytes

clone of cells
1 of 43
Lymphocytes produce antibodies against anything the body ?
detects as foreign
2 of 43
The antibodies are specific to binding on one protein?
antigen
3 of 43
PROCESS
---------------
Firstly a mouse is ?
injected
4 of 43
Injected with what?
a specific antigen
5 of 43
Then the lymphocyte will do what?
produce antibodies against the antigen
6 of 43
Then we will collect?
the lymphocytes from the mouse
7 of 43
What is a problem with the lymphocytes?
they will not divide by mitosis
8 of 43
Therefore well will do what to the lymphocyte?
fuse it
9 of 43
Fuse it with what?
a tumour cell
10 of 43
Why a tumour cell?
they are very good at dividing by mitosis
11 of 43
This produces a what cell?
hybridoma cell
12 of 43
A hybridoma cell can do what two things?
divide by mitosis
produce antibodies
13 of 43
Then we will select what?
one single hybridoma cell
14 of 43
Which produces what?
the desired antibody
15 of 43
And we will allow it to what?
divide by mitosis
16 of 43
This will form a _____ of identical______cells?
clone
hybridoma
17 of 43
A large amount of these are then what?
collected and purifies
18 of 43
USES
-------
One use of monoclonal antibodies is with what?
pregnancy
19 of 43
The hormone___ is made in the early stages of pregnancy?
HCG
20 of 43
Tiny amounts are passed in?
urine
21 of 43
The monoclonal antibodies are then _______ to the end of a _______ ____ which are _______ to detecting___??
attached to the end of a pregnancy stick which are specific to detecting HCG
22 of 43
The results slide also has some more _______ in _____ positions?
antibodies in fixed positions
23 of 43
If pregnant, the urine will ____ __ the stick carrying the ____ with it and will bind to the _______ in the _____ area showing a _____ test?
move up the stick carrying the HCG with it and will bind to the antibodies in the results area showing a positive test
24 of 43
If you are not pregnant, the urine will ______ to move up the stick and the test will be ____?
continue
negative
25 of 43
Cancer Diagnosis and Treatment
------------------------------------
Diagnosis
Firstly a specific what is injected?
antibody
26 of 43
Cancer cells have ______ on their cell _______.
These are called what?
antigens of their cell membrane
called tumour markers
27 of 43
These antibodies will then bind with what?
cancer cells
28 of 43
And do what to them?
clump them together
29 of 43
This makes it what to identify?
easier
30 of 43
Treatment

These antibodies will also have what attached to them?
a radioactive/ toxic drug attached to them
31 of 43
These drugs will stop what?
cancer cells from growing and dividing
32 of 43
Since they are specific, this prevents what?
harm to other healthy cells
33 of 43
Monoclonal antibodies can be used to research to find?
specific substances
34 of 43
They can bind to _______ and other chemicals in the _____ to measure their ____?
bind to hormones and other chemicals in the blood to measure their level
35 of 43
Test blood samples for certain?
pathogens
36 of 43
Or locate specific_______ in a ____ or ____?
locate specific molecules in a cell or tissue
37 of 43
Firstly what is made?
monoclonal antibodies which are specific to the molecule your looking for
38 of 43
Antibodies are then bound to what?
a flourescent dye
39 of 43
Molecules present, the antibody?
will attach to them
40 of 43
Then they can be detected?
using the dye
41 of 43
An advantage?
treat cancer without harming other healthy cells
42 of 43
Disadvantage?
side effects like fever vomiting
43 of 43

Other cards in this set

Card 2

Front

Lymphocytes produce antibodies against anything the body ?

Back

detects as foreign

Card 3

Front

The antibodies are specific to binding on one protein?

Back

Preview of the front of card 3

Card 4

Front

PROCESS
---------------
Firstly a mouse is ?

Back

Preview of the front of card 4

Card 5

Front

Injected with what?

Back

Preview of the front of card 5
View more cards

Comments

No comments have yet been made

Similar Biology resources:

See all Biology resources »See all Medicine and drugs resources »