Biology-B2-Investigating Enzymatic Reactions (Practical)

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  • Created by: tonisha_
  • Created on: 05-11-22 19:16
Amylase breaks down ______to _____ _____?
starch to simple sugars
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In this we will investigate the effect of pH on which enzyme?
amylase
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STEPS
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Firstly, use ____ and place one drop of it into each ____ of a ______ tile?
use iodine and place 1 drop of it into each well of a spotting tile
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Then get _ test tubes?
3
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One should be filled with _cm cubed of ______ solution
One should be filled with _cm cubed of _______ solution
One should be filled with _cm cubed of pH 5 _____ solution?
1-2cm cubed of starch solution
2-2cm cubed of amylase solution
3-2cm cubed of pH 5 buffer solution
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What is buffer solution used to control?
pH levels
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Then where do we place all three test tubes?
into a water bath
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At __ degrees Celsius using a _______?
at 30 degrees using a thermometer
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Then we leave it in the water bath for how long?
10 mins
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For the solutions to reach the correct?
temperature
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Then what do we do to all the solutions?
combine them into one test tube
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Then stir it with a ?
stirring rod
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And start a?
stopwatch
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After __ seconds use the stirring rod to transfer 1 drop of _______ into a ____ in the ____ ____ containing _____?
after 30 seconds use the stirring rod to transfer 1 drop of the solution into a well in the spotting tile containing iodine
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If starch is present what is the colour change?
orange to blue black
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Then take a sample every ___ seconds and continue until?
every 30 s and continue until iodine remains orange
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If the iodine stays orange this means that there is no?
starch
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This means that the reaction is?
complete
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Now look at the ______ and record the ____?
look at the stopwatch and record the time
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Repeat this whole investigation for different what?
pH levels
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e.g.?
pH 6,7,8
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What is the Independent variable?
the pH level
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Dependent Variable?
time taken for amylase to break down starch
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Control?
time between each sample

volume of solutions
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PROBLEMS
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One problem is that we only take a sample every ___ seconds?
30
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This only gives us an _______time for reaction to?
approximate time for reaction to complete
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How can we address this?
by sampling every 10 s
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Another problem is that we only stop when?
iodine stays orange
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This is a problem since sometimes it is not?
obvious
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Since colour change can be?
gradual
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How can we address this?
by getting several people to look and decide
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Other cards in this set

Card 2

Front

In this we will investigate the effect of pH on which enzyme?

Back

amylase

Card 3

Front

STEPS
------
Firstly, use ____ and place one drop of it into each ____ of a ______ tile?

Back

Preview of the front of card 3

Card 4

Front

Then get _ test tubes?

Back

Preview of the front of card 4

Card 5

Front

One should be filled with _cm cubed of ______ solution
One should be filled with _cm cubed of _______ solution
One should be filled with _cm cubed of pH 5 _____ solution?

Back

Preview of the front of card 5
View more cards

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