Biology-B1-Microscopy+Practical 0.0 / 5 ? BiologyBiotechnology and the use of microbes in industryGCSEAQA Created by: tonisha_Created on: 26-06-21 09:24 What do light microscopes use to view a specimen? light 1 of 38 What does an electron microscope use to form an image? electrons 2 of 38 How many centimetres are in a metre? 100cm 3 of 38 How many millimetres are in a centimetre? 10mm 4 of 38 How many micrometres are in a metre? 1,000,000um (million) 5 of 38 How many nanometres are in a metre? 1,000,000,000nm (billion) 6 of 38 what are the disadvantages of a light microscope? limited magnification limited resolution 7 of 38 What are the advantages of an electron microscope? greater magnification greater resolution 8 of 38 What is the resolution? how clear an image is 9 of 38 What is the magnification? how far you can zoom in on an image 10 of 38 What is the equation for magnification? magnification=image size/actual size 11 of 38 What is the equation for image size? image size=actual size X magnification 12 of 38 What is the equation for actual size? actual size=image size/magnification 13 of 38 What is an acronym for this equation? I AM 14 of 38 PRACTICAL ------------------ What is the first stage of this practical? preparing the slide 15 of 38 Firstly, add a drop of _____ onto the _____ of the clean glass slide? add a drop of water onto the middle of the clean glass slide 16 of 38 Then cut up an ______ into _____ pieces and use _____ to peel off _ layer(s) of ________ tissue? cut up an onion into small pieces and use tweezers to peel off 1 layer of epidermal tissue 17 of 38 Why is a thin layer of epidermal tissue used? to help see individual cells and allow light to penetrate through 18 of 38 Then place the ______ _____ onto the slide where the _____ is. place the epidermal tissue onto the slide where the water is 19 of 38 Add a drop of ______ solution to ______ and add _____ to the structures in the onion? add a drop of iodine solution to highlight and add colour to 20 of 38 Place a _____ ____ over the slide by slowly lowering it? place a cover slip 21 of 38 Try not to get any ? air bubbles 22 of 38 As this would? obstruct the view 23 of 38 The second stage of this is to? use the light microscope 24 of 38 Firstly ____ the slide onto the ____? clip the slide onto the stage 25 of 38 Then select the ______ powered ______ _____? select the lowest powered objective lens 26 of 38 Use the _____ ______ ___ to move the stage up just _____ the objective lens? use the coarse adjustment knob to move the stage up just below the objective lens 27 of 38 Look down in the _______ and adjust the ______ ______ ______ until image is roughly in focus Look down in the eyepiece and adjust the coarse adjustment knob until image is roughly in focus 28 of 38 Then use the _____ knob to get a more ____ ___. use the focus knob to get a more clear image 29 of 38 If we want to see the slide in a greater magnification what do we do? swap to a higher powered objective lens 30 of 38 What is the third stage? Drawing the observations 31 of 38 You must use a ? pencil 32 of 38 You must not ? sketch 33 of 38 Your drawing must be in ? proportion 34 of 38 At the end you should also include a what and do what to the diagram? include a title and label the diagram 35 of 38 How do you convert from cm to mm? x10 36 of 38 How do you convert from mm to micrometres? x1000 37 of 38 micrometres to nanometers? x1000 38 of 38
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