Biology B1 1.7 Growing and investigating bacteria.

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Where can bacteria be grown?
On agar jelly.
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When growing cultures, why must all the materials and equipment used be sterilised?
To ensure that unwanted microorganisms do not infect the culture.
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What can uncontaminated cultures be used for?
To investigate the effect of antibiotics and disinfectants on the bacteria.
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Apart from from sterilising materials and equipment, how else can bacterial culture be kept pure?
Air contains microorganisms that can contaminate culture, therefore the microorganisms from the air must be killed or prevented from entering the culture.
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What kind of cultures can be used for laboratory investigations?
Pure cultures of non-pathogenic (safe) bacteria.
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What can a culture of microorganisms bused to find?
The effect of antibiotics on bacteria.
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Why are strict health and safety procedures required when investigating uncontaminated cultures of microorganisms?
To protect yourself and others.
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Where may contamination of cultures come from?
Your skin, the air, the soil or water around you.
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What may happen if the culture is contaminated?
Other bacteria could grow, including pathogens.
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What do you need to culture (grow) microorganisms in a laboratory?
A liquid or gel (a culture medium - agar jelly), warmth and oxygen.
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What does a culture medium contain?
Nutrients, carbohydrate as an energy source, various minerals and sometimes other chemicals.
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How warm should the culture be kept?
It should be incubated at 25 degrees C in school laboratories and at 35 degrees C in industry.
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What do you need to do to keep the culture pure?
Kill all the bacteria on the equipment and prevent microorganisms from the air getting into the equipment.
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How do you kill all the bacteria on the equipment?
Pass metal loops through a flame and boil solutions and agar.
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Why do industrial laboratories use 35 degrees C instead of 25 degrees C as in schools?
The bacteria will grow at a faster rate for industry.
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How do you make sure that unwanted microorganisms do not contaminate a pure culture of bacteria?
Heat all equipment, boil solutions and cover Petri dishes or flasks.
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Card 2

Front

When growing cultures, why must all the materials and equipment used be sterilised?

Back

To ensure that unwanted microorganisms do not infect the culture.

Card 3

Front

What can uncontaminated cultures be used for?

Back

Preview of the front of card 3

Card 4

Front

Apart from from sterilising materials and equipment, how else can bacterial culture be kept pure?

Back

Preview of the front of card 4

Card 5

Front

What kind of cultures can be used for laboratory investigations?

Back

Preview of the front of card 5
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