Biology

  • Created by: alisha
  • Created on: 29-10-12 15:59
What does resolution mean?
to be able to see two distinct points seperately
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what is the maximum magnification of a light microscope
x1500
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What is the maximum resolution for a light microscope is
200nm
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what does staining do ?
allows the specimen to be seen and contrasts the colours
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what does Sectioning do?
specimens are put in wax and sections are then cut without damaging the structure of a specimen
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What are specimens?
organisims that can be viewed under a light microscope
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What is the highest resolution for a electic microscope
0.20nm
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what is the highest manification possible of a transmission electron microscope
x500 000
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The highest magnification of a scanning electron microscope
x100 000
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How many types of electron microscopes are there what are their names
There are 2 TEM: transmission electron microscope and SEM: Scanning electron microscope
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name the limitations of an electron microscope
has to be placed in a vacum because electron beams are deflected by molecules in the air, very expensive, need high degree and skill to use it
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what is the purpose of a condenser
controls amount of light going through the specimen
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formula for calculating the magnification?
magnification=image size/ actual size
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comparison of a light microscope to a electron microscope
Cheap to purchase,Cheap to operate.specimens can be living or dead, stains make cells more visible.vacum not required
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comparision of a electron microscope to a light microscope
expensive to buy,expensive to produce elctron beam,vacum required,specimens need to be dead because they need to be put in plastic and be put in a vacum.
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what is the maximum magnification of a light microscope

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x1500

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What is the maximum resolution for a light microscope is

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what does staining do ?

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what does Sectioning do?

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