Required Practical Notes

REQUIRED PRACTICAL NOTES 

1. Investigation into the effect of a named variable on the rate of an enzyme-controlled reaction

- Effect of temperature on 'rate of reaction' catalysed by the enzyme 'trypsin'

Notes 

• Buffer added - keep pH constant 
• Optimum temperature 
• Denaturing of enzymes
• Could have human error in visual of the cross
• Higher temp = more kinetic energy 

2. Preparation of stained squashes of cells from plant root tips; set-up and use of an optical microscope to identify the stages of mitosis in these stained squashes and calculation of a mitotic index

- Investigating mitosis

Notes

• When squashing do not roll fingers - move chromosomes in the cell, cannot see the mitosis, muddle the chromatid 
• Easily can go wrong 
• Wear goggles - harmful chemicals could effect eyes and eyesight 

3. Production of a dilution series of a solute to produce a calibration curve with which to identify the water potential of plant tissue

- Water potential of potato 

Notes 

• Do more readings around the value of the water potential 
• Mass…