Factors Affecting Enzyme Activity

Temperature

The rate of an enzyme controlled reaction increases when the temperature increases. More heat means more kinetic energy so molecules move faster. This increases the likelihood that the substrate will collide with the active site. The energy of the collisions also increase so is more likely to result in a reaction. The rate of reaction increases until it meets optimum temperature. If the temperature gets too high the reaction stops as the enzyme molecules vibrate more. This vibration can break the bonds so the active site changes shape and the substrate can no longer bind. This is when the enzyme is denatured. 

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