1. Vessel sterilised using high pressure steam to kill any microbes, preventing contamination.
2. Sterile nutrient medium added.
3. Pure Penicillium notatum culture added.
4. Fungus provided with suitable conditions for growth:
- Sterile air intoduced to provide oxygen for aerobic respiartion. Prevents contamination from airborne microbes.
- Culture heated to optimum temperature for growth, mainting by cooling water jacket that removes excess heat from fungal respiration.
- sparging ring/paddles thoroughly mixes the culture and nutrients by forced aeration.
- once glucose depleted, fungus enters stationary and penicillin is produced.
- culture fluid drained and filtered to remove the mycelium and the filtrate is purified to extract the penicllin.
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