BY4 - Microbiology
- Created by: beth-marie2511
- Created on: 11-06-16 16:04
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- BY4 - Microbiology
- Obligate Aerobe
- Micro-organisms that grow/divide in the presence of oxygen
- Obligate Anaerobe
- Micro-organisms that grow/divide in the absence of oxygen
- Facultative Anaerobe
- Micro-organisms that can grow/divide with or without oxygen
- Aerobic respiration is more beneficial
- Micro-organisms that can grow/divide with or without oxygen
- Fermenters
- AIM: To grow a culture of micro-organisms on a large scale to produce useful products.
- Large stainless steel tank
- Maximum efficiency is created by there being no competition from other organisms
- Pure culture of an organism is needed to form and harvest a pure product
- Controlled conditions
- Penicillin Producion
- Tank is sterilised and a pure culture of PENICILLIUM NOTATUM is added to a fermenter
- Sterile nutrient medium is added to the tank through a filter.
- Glucose is (carbon source) is added and needed for respiration.
- Ammonia (nitrogen source) is used in protein synthesis
- Nutrients and micro-organisms are mixed by stirrers/ paddles
- Temperature is controlled by a cooling jacket
- It also removes excess heat produced by respiration.
- Monitored by a temperature probe
- pH is controlled by a specific pH buffer from the acid/base reservoir
- Forced aeration is needed for growth
- Air comes out through a sparger
- Culture fluid is filtered - mycelium is separated from penicillin
- Penicillin is chemically purified to form the antibiotic
- Obligate Aerobe
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