Gel electrophoresis

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  • Gel electrophoresis
    • Sample obtained
    • PCR used to amplify DNA
    • Fluorescent tag added to all DNA fragments
      • So it can be viewed under UV light
    • DNA mixture placed in a well in agar in a tank
      • Agar covered in buffer solution so it conducts electricity
      • Anode placed near wells, Cathode placed at opposite end
    • Electrical current passed through gel
      • DNA negatively charged so will travel towards cathode
    • DNA separates by size
      • Smaller fragments are lighter so they'll travel faster and further
    • DNA fragments can be viewed as bands under UV light
    • DNA transferred to a nylon membrane by solution drawn up through the gel.
    • Membrane placed in bag with DNA probe

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