Gel electrophoresis
- Created by: tumblrgirl96
- Created on: 04-05-15 13:54
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- Gel electrophoresis
- Sample obtained
- PCR used to amplify DNA
- Fluorescent tag added to all DNA fragments
- So it can be viewed under UV light
- DNA mixture placed in a well in agar in a tank
- Agar covered in buffer solution so it conducts electricity
- Anode placed near wells, Cathode placed at opposite end
- Electrical current passed through gel
- DNA negatively charged so will travel towards cathode
- DNA separates by size
- Smaller fragments are lighter so they'll travel faster and further
- DNA fragments can be viewed as bands under UV light
- DNA transferred to a nylon membrane by solution drawn up through the gel.
- Membrane placed in bag with DNA probe
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