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Emily Summers

Electrophoresis Separation of different lengths of DNA fragments in a mixture is
achieved as negatively charged fragments move towards the positive electrode (anode)
from the cathode, shorter fragments pass more freely through the gel so move further in
the fixed time period.

1. Treat DNA samples with restriction…

Page 2

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Emily Summers

DNA probes are short, single stranded pieces of DNA which are about 5080 nucleotides
long, that is complementary to a section of the DNA being investigated.

Labelling the Probe:

Using a radioactive marker 32

P in the phosphate groups forming the strand, so the
location can be revealed…


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