- When the concentration of a substrate is increased, the no. of substrate atoms, molecules or ions in a particular area or volume increases. This means there will be more frequent, successful collisions between subtrate and active site, so there will be an increase in the formation of enzyme-substrate complexes (rate of reaction increases)
Maximum = Vmax --> all the active sites are occupied by substrate particles. No more enzyme-substrate complexes can be formed until products are released from active sites
- ^ The only way to increase the rate of reaction in this circumstance would be to add more enzyme or increase the temperature
If the concentration of an enzyme is increased, more active sites are available. This means it becomes a limiting factor again and increasing means the reaction rate will rise until the new Vmax is reached
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