Temperature
Enzymes work the fastest they can at their optimum temperature. Below this at around 0 degrees the enzyme works, but incredibly slowly. As temperature increases, the substrate and enzyme molecules gain more kinetic energy and so move faster resulting in more collisons and more products and so the rate of the reaction increases. Above the optimum temperature, the enzymes begin to denature, meaning the hydrogen bonds in the tertiary strcuture begin to break, this causes the active site to lose its shape and so the substrate will no longer fit and no more product will be produced.
PH
As with temperature, enzymes also have an optimum PH. If the PH strays too far from the optimum (this margin can be very narrow) this changes the charge on the active site as a incorrect PH disrupts the charges on the hydrogen and ionic bonds within the molecule, changing the shape of the active site, so no enzyme-substrate complexes can be formed, resulting in no product.
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