Immobilised enzymes are enzymes that are attached to an inert, insoluble material (eg. calcium alginate). Immobilised enzymes can be used to make lactose intolerant/cat milk through catalysing the breakdown of lactose to glucose and galactose.
Advantages
- can catalyse the same reaction many times
- binding makes enzymes more stable and less likely to denature
- purification is not necessary
Disadvantages
- requires extra time, equipment and work
- may be reduction in reaction time
- cannot be used if one of the substrates is insoluble
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