Uncontaminated cultures of microorganisms are required for seeing the effect of disinfectants and antibiotics for this:
1. Petri dishes and equipment must be sterilised to kill microorganisms.
2. Innoculating hoops used to transfer microorganisms must be passed through a flame to kill unwanted bacteria.
3.The lid of a Petri dish should be secured with tape to prevent contamination from the air.
In labs cultures should be incubated at a maximum of 25'c which reduces the risk of harmful pathogens growing.
In industrial conditions higher temperatures can produce more rapid growth.
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