Microscopy Unit 1!

Unit 1

Microscopy

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Microscopy
Resolution: How detailed the image is
Magnification: How large the image can be made
MAGNIFICATION = SIZE OF IMAGE ÷ ACTUAL SIZE OF SPECIMEN
Convert How
mm to µm ×1000
mm to nm ×1,000,000
µm to nm ×1000
µm to mm ÷1000
nm to mm ÷1,000,000
nm to µm ÷1000
Light Microscope Electron Microscope
Light Electrons
Lower Resolution Higher Resolution
Longer Wavelength Shorter Wavelength
TEM SEM
Use electromagnet to focus a beam of Scan a beam of electrons across specimen
electrons
Beam is transmitted through specimen Knocks off electrons from the specimen,
which gathered in a cathode ray tube to
form an image
Denser part of specimen absorbs more Can be 3D
electrons making the image darker Images show specimen surface
Give higher resolution images Can be used on thick specimen
Can only be used on thin specimens Lower Resolution

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