Magnification = how many times larger the image is than the actual size of the object being viewed.
Resolution = the ability to see individual objects as separate entities. The resolution of a microscope determines the amount of detail that can be seen - higher the resolution, the more visible details.
Unit conversions:
100 nanometres (nm) = 1 micrometre (μm)
1000 micrometres (μm) = 1 milimetre (mm)
100 milimetres (mm) = 1 centimetre (cm)
Calculation for magnification:
magnification = (size of image) / (actual size of object)
Using a graticule to calibrate a light microscope:
- Eyepiece graticule = a glass disc marked with a fine scale of 1 to 100.
- Stage micrometer = a microscope slide with a very accurate scale in micrometres (μm) engraved on it. Usually 100 divisions = 1mm, so 1 division = 10 μm.
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