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Biology F215

Gene Technology

Key Words


Key words Definition

Allotransplantation Transplantation of organs between individuals of the same species, for
example transplantation of a human heart into another human.

Comparative genome mapping The comparison of DNA sequences coding for the production of proteins and
regulatory sequences in the genomes of…

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Golden RiceTM A variety of rice that is genetically engineered to produce large amounts of the
vitamin A precursor beta-carotene.

Intron A length of non-coding DNA found within a gene that is transcribed into mRNA
but spliced (cut) out before translation

Polymerase Chain Reaction (PCR) Process for the amplification (making…

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What is gene technology?

The term gene technology refers to the application of techniques that enable us to isolate and manipulate
sections of DNA

What are the techniques and applications of gene technology?




What is a genome?

The genome of an organism is the entire DNA sequence of that organism…

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By analysing the DNA of individuals of the same species we are able to see for example genes linked to a disease
or if they are a carrier for a mutant allele which can cause a genetic disease
o It also allows us to trace the migration of early humans…

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Assemble all the BAC sequences using a computer program
that compares the overlapping regions



Put the following into the correct order for the sequencing of a genome




What are the regulatory functions of non-coding DNA?

Non coding DNA may encode for short antisense RNAs
that are able to bind to…

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o They plan to use siRNA as a drug to silence these genes and prevent their expression and stop the
tumour from growing

Why are silent genes important in cellular division?

Differentiation of cells requires some genes to be switched on and others for the alternative pathways of
development to…

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o This movement is due to solubility in a solvent or a pd across the
gel

What is the DNA binding dye required for?

The DNA binding dye is used to reveal the positions of the DNA bands
o As well as allowing the gel and the final positions of…

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The gel is then blotted and probed for the short tandem repeat (STR)
o Up to eight STR's are examined for and this produces a unique profile or DNA
fingerprint that can be compared with the sequence of other individuals

How can DNA profiling be used to determine the paternity…

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What is the Polymerase Chain Reaction?

PCR is a technique that can be used to
increase (amplify) the number of copies
of a DNA fragment
o It is a type of `artificial DNA
replication'
It relies on separation
of the DNA strands
Each strand
acts as the
template for
the…

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What are the comparisons between DNA replication and PCR?

Both of these processes rely on the separation of two
strands of DNA
o In both processes complementary base
pairing rules are used for the formation of the
new strand between the incoming nucleotide
and the single template strand
Finally both…

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cmdw1

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Brilliant resource. Thanks

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