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How enzymes work ­ Reference pages 2-5 knowledge booklet
ACTIVITY 1
Read the information on page 25 about how enzymes work in silence and highlight all
the key scientific words.…read more

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CO 2 and H
20 at the ambient
temperature (the temperature of the surrounding environment) alone.
.........................................................................................................................................................
..........................................
2. Would the paper burning release more, less or the same energy as the
decomposition? Explain your answer.
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..........................................…read more

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ACTIVITY 6 -Match the substrate with the enzyme.
Enzyme Substrate
DNA ligase 1
DNA
Trypsin 2
Protein
ATPase 3
ATP
Amylase 4
Starch
Lipase 5
Lipids
Galactosidase 6
lactose
Maltase 7
maltose
ACTIVITY 7 - Reference pages 4-5 knowledge booklet
Now read, remember and recite the following topics:
1) How enzymes work
2) The induced fit model
3) The digestion of starch
4) The digestion of proteins
Test your partner, see how much they can remember and vice versa.…read more

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Label on the diagram the active site of the enzyme.[1]
(b) Write the letter of the molecule that is most likely to be the substrate for this enzyme.
.........................................................................................................................[1]
(c)
Use the information in the diagram to explain e
nzyme specificity.
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.........................................................................................................................[3]
(d) One hypothesis of the mechanism of enzyme action is the `lock and key' hypothesis.
Another hypothesis, the `induced fit' hypothesis, involves the enzyme changing
shape slightly to allow the substrate to fit perfectly. The substrate also changes
shape slightly.…read more

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Q 3 The graph
shows the energy
changes which take
place during the
reaction in which
hydrogen peroxide is
converted to water
and oxygen.
Use the graph to
explain why
(i) hydrogen peroxide breaks down at a lower temperature when catalase is
present than when it is not present
...........................................................................................................................
...........................................................................................................................(1)
(ii) test tubes containing reactants became warmer when the reaction was taking
place.
...........................................................................................................................
...........................................................................................................................…read more

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Questions:
1. Which graph, A or B, displays the fastest enzyme catalysed reaction? Graph A
A
Explain the reasons for your choice.
Steepest gradient, reaches maximum in less time
2.…read more

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ACTIVITY 10 -Reference pages 6-9 knowledge booklet
Complete the following table:
Is there a change in trend?
Describe and explain the initial
Graph If so, when does this
trend
occur?
Description
There is a peak of activity at 37oC
Initially, enzyme activity increases as after which enzyme activity
temperature increases decreases;
Explanation
Increase in temperature increases the kinetic
energy of the enzymes and substrates;
There are more successful enzyme and substrate
collisions/ES complexes formed;
Description There is a peak of activity at a pH of…read more

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Description The maximum rate of reaction is
reached at the saturation point/Vma
Initially, enzyme activity increases as substrate
concentration increases
Explanation
With more substrates in the solution, there are
more successful enzyme and substrate
collisions/ES complexes formed;
Description The maximum rate of reaction is
Initially, enzyme activity increases as enzyme reached when substrate
concentration increases concentration becomes limiting
Explanation
With more enzymes in the solution, there are
more successful enzyme and substrate
collisions/ES complexes formed;
9…read more

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Q 1. The fungus, yeast, contains the enzyme catalase.
Catalase speeds up the decomposition of hydrogen peroxide, a toxic metabolic product, to
oxygen and water.
2 H2O2 2 H2O + O2
A student decided to investigate the activity of catalase by measuring the gas produced in
a given time. The total volume of gas collected was recorded every 20 seconds.…read more

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