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Application booklet



1

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How enzymes work ­ Reference pages 2-5 knowledge booklet
ACTIVITY 1



Read the information on page 25 about how enzymes work in silence and highlight all
the key scientific words. Find the terms that match the following definitions:
Metabolism All the chemical reactions in an organism needed for life
Anabolism…

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c) how the paper in the compost heap was broken down to CO 2 and H
20 at the ambient

temperature (the temperature of the surrounding environment) alone.




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2. Would the paper burning release more, less or the same energy as the
decomposition? Explain your answer.…

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ACTIVITY 6 -Match the substrate with the enzyme.

Enzyme Substrate

DNA ligase 1
DNA

Trypsin 2
Protein

ATPase 3
ATP

Amylase 4
Starch

Lipase 5
Lipids

Galactosidase 6
lactose

Maltase 7
maltose


ACTIVITY 7 - Reference pages 4-5 knowledge booklet
Now read, remember and recite the following topics:
1) How…

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(a) Label on the diagram the active site of the enzyme.[1]

(b) Write the letter of the molecule that is most likely to be the substrate for this enzyme.

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(c)
Use the information in the diagram to explain e
nzyme specificity.

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(d) One hypothesis of…

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Q 3 The graph
shows the energy
changes which take
place during the
reaction in which
hydrogen peroxide is
converted to water
and oxygen.

Use the graph to
explain why

(i) hydrogen peroxide breaks down at a lower temperature when catalase is
present than when it…

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Questions:

1. Which graph, A or B, displays the fastest enzyme catalysed reaction? Graph A
A

Explain the reasons for your choice.



Steepest gradient, reaches maximum in less time



2. Why do both reactions plateauat the same total amount of maltose?


Same amount of starch at the beginning, broken down…

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ACTIVITY 10 -Reference pages 6-9 knowledge booklet
Complete the following table:

Is there a change in trend?
Describe and explain the initial
Graph If so, when does this
trend
occur?
Description
There is a peak of activity at 37oC
Initially, enzyme activity increases as after which enzyme activity
temperature increases…

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Description The maximum rate of reaction is
reached at the saturation point/Vma
Initially, enzyme activity increases as substrate
concentration increases

Explanation
With more substrates in the solution, there are
more successful enzyme and substrate
collisions/ES complexes formed;


Description The maximum rate of reaction is
Initially, enzyme activity increases as enzyme…

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Q 1. The fungus, yeast, contains the enzyme catalase.

Catalase speeds up the decomposition of hydrogen peroxide, a toxic metabolic product, to
oxygen and water.

2 H2O2 2 H2O + O2


A student decided to investigate the activity of catalase by measuring the gas produced in
a given time. The…

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