In Vitro Cloning (PCR)

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In Vitro Cloning (PCR)


  • can produce lots of DNA
  • only replicates the DNA fragment of interest (target gene) so you don't have to isolate the DNA fragment from host DNA or cell components
  • fast process, cloning millions of copies of DNA in a few hours


  • cannot produce mRNA or protein
  • can only replicate a small DNA fragment
  • expensive if you want to produce a lot of DNA


DNA produced isn't modified. This can be an advantage if you don't want modified DNA or a disadvantage if you do


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