DNA Technology Mind Map part 1

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  • DNA Technology
    • Making a protein by gene transfer and in vivo cloning.
      • 1) Produce DNA Fragments - Use Reverse Transcriptase or Restriction Endonucleases
        • 2) Insert DNA Fragment into Vector
          • 3) Transfer vector into host cell - In Vivo cloning Plasmid and Bacterial Cell mixed together with Ca2+
            • 4) Identify the cells that have taken up the plasmid by GENE MARKERS = Antibiotic GM (replica plating)Fluorescent GM or Enzyme Markers
    • Copying DNA Fragments - In vitro gene cloning
      • Polymerase Chain Reaction - PCR 1) separation of the DNA strand 2) addition of primers 3) synthesis of DNA
    • Genetic Fingerprinting
      • 1) Extraction 2) Digestion 3) Separation 4) Hybridisation 5) Development
        • 1) DNA is extracted from the sample
          • 2) Restriction Enzyme cut DNA
            • 3) Gel Electrophoresis
              • 4) Fragments transferred from gel to nylon membrane (southern blotting)
                • 5) DNA probes added
                  • 6) Membrane placed on X- Ray film and film developed

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