DNA Technology Mind Map part 1
- Created by: Chloe Sadler
- Created on: 02-04-14 16:23
View mindmap
- DNA Technology
- Making a protein by gene transfer and in vivo cloning.
- 1) Produce DNA Fragments - Use Reverse Transcriptase or Restriction Endonucleases
- 2) Insert DNA Fragment into Vector
- 3) Transfer vector into host cell - In Vivo cloning Plasmid and Bacterial Cell mixed together with Ca2+
- 4) Identify the cells that have taken up the plasmid by GENE MARKERS = Antibiotic GM (replica plating)Fluorescent GM or Enzyme Markers
- 3) Transfer vector into host cell - In Vivo cloning Plasmid and Bacterial Cell mixed together with Ca2+
- 2) Insert DNA Fragment into Vector
- 1) Produce DNA Fragments - Use Reverse Transcriptase or Restriction Endonucleases
- Copying DNA Fragments - In vitro gene cloning
- Polymerase Chain Reaction - PCR 1) separation of the DNA strand 2) addition of primers 3) synthesis of DNA
- Genetic Fingerprinting
- 1) Extraction 2) Digestion 3) Separation 4) Hybridisation 5) Development
- 1) DNA is extracted from the sample
- 2) Restriction Enzyme cut DNA
- 3) Gel Electrophoresis
- 4) Fragments transferred from gel to nylon membrane (southern blotting)
- 5) DNA probes added
- 6) Membrane placed on X- Ray film and film developed
- 5) DNA probes added
- 4) Fragments transferred from gel to nylon membrane (southern blotting)
- 3) Gel Electrophoresis
- 2) Restriction Enzyme cut DNA
- 1) DNA is extracted from the sample
- 1) Extraction 2) Digestion 3) Separation 4) Hybridisation 5) Development
- Making a protein by gene transfer and in vivo cloning.
Comments
No comments have yet been made