respiration investigation and evidence for chemiosmosis
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?- Created by: vezting
- Created on: 26-04-16 17:51
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- respiration - investigation and evidence for chemiosmosis
- investigation
- 1: a respirometer is used to measure the rate of oxygen is taken up
- 2:each tube contains soda lime which absorbs CO2
- 3:control tube sat up exactly the same with glass beads
- 4: syringe used to set up fluid in manometre to a known level
- 5: respirometre left for a set period of time e.g. 20 minutes
- 6:there will be a decrease in volume of air due to o2 consumed by the organism
- 7: decrease in volume reduces pressure in tube and liquid will move towards the tube
- 8: distance moved in a given time is measure
- 9: variable shpould be controlled e.g. temp and volume of soda lime
- 10: repeat and take a mean to get a reliable result
- evidence for chemiosmosis
- pH of inter membrane space: it has lower pH than matrix meaning more acidic implying more H ions showing proton gradient
- atrtificial vesicles: created from phospholipid bi layer to represent inner mitochondrial matrix and pproton pumps taken from bacteria and atp synthase added to membrane
- proton pumps activated by light, pH inside vesicles decreases, due to protons pumped into vesicles
- when adp and Pi added ATP was synthesised showing a proton gradient used to syntheise ATP
- proton pumps activated by light, pH inside vesicles decreases, due to protons pumped into vesicles
- mitochondria placed in pH 8, when ADP and Pi added, there was no ATP synthesised when placed in pH 4 prototn gradient was created and atp synthesised
- uncouplers are substances that destroy proton graident across inner mitochindrial membrane when NAD ADP and Pi added, not ATP, meaning proton gradient is needed
- investigation
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