PCR 3 step cycle
- Created by: Hannah Gilmore
- Created on: 15-12-12 13:22
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- PCR
- 1. Heat to separate strands
- Heat to 95oC to separate two DNA strands and H Bonds.
- 2. DNA Primers Added
- Mixture cool to 53oC to let primers attach to start of each DNA strand.
- Primer purpose: STOP Rejoining, Seleect are to be COPIED, Signal action of DNA Polymerase
- Primers easy to identify - radioactive
- Mixture cool to 53oC to let primers attach to start of each DNA strand.
- 3.Use of heat-stable DNA polymers - extend primers
- Mixture heates to 73oC - thermostable DNA Pol. copies each strand.
- Heat DNA Pol. add free nucleotides to expose strand - attach according to bas pair rules.
- A-T, G-C
- Heat DNA Pol. add free nucleotides to expose strand - attach according to bas pair rules.
- Mixture heates to 73oC - thermostable DNA Pol. copies each strand.
- 1. Heat to separate strands
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