Measuring bacterial growth
- Created by: Ellie0o0o
- Created on: 26-04-15 12:27
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- Measuring bacterial growth
- Counting cell numbers
- Direct microscope counting
- Chequered cover slip can be used like a quadrat
- DISADVANTAGE: Cannot differentiate between living and dead cells
- Viable counts
- Specifically living cells
- Spread agar plates with bacteria. Each individual will grow into a colony. Count the colonies
- Often a number of dilutions need to take place before the concentration is suitable for spreading
- Turbidity
- INDIRECT measure of cell mass
- Less accurate than other measures
- Quick and easy
- Spectrophotometer measures the amount of UNSCATTERED light
- Unscattered light is compared to an OD curve
- Counting cell numbers
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