Measuring bacterial growth

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  • Created by: Ellie0o0o
  • Created on: 26-04-15 12:27
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  • Measuring bacterial growth
    • Counting cell numbers
      • Direct microscope counting
      • Chequered cover slip can be used like a quadrat
      • DISADVANTAGE: Cannot differentiate between living and dead cells
    • Viable counts
      • Specifically living cells
      • Spread agar plates with bacteria. Each individual will grow into a colony. Count the colonies
      • Often a number of dilutions need to take place before the concentration is suitable for spreading
    • Turbidity
      • INDIRECT measure of cell mass
      • Less accurate than other measures
      • Quick and easy
      • Spectrophotometer measures the amount of UNSCATTERED light
        • Unscattered light is compared to an OD curve


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