Microbiology lecture 7 0.0 / 5 ? BiologyHow do we cultivate bacteria?UniversityNone Created by: willmiddlehamCreated on: 03-04-15 16:26 What are the two groups of nutrients bacterial cells need? Macronutrients, micronutrients 1 of 10 In what amounts are macronutrients required? C - 50%, O - 17%, N - 13%, P - 2.5% 2 of 10 How do you calculate the final cell number? N = No(initial cell no.) x Z^n(no. of generations) 3 of 10 How do you measure bacterial growth by dilutions? Colony forming units extrapolated to give cell numbers in original culture. Only measure viable cells. Underestimates for cells in chains or clusters 4 of 10 What is cytoplasmic pH? pH 7 5 of 10 What process can cause pH to change often? Batch, chemostat growth. So need a buffer (Na2HPO4 and KH2PO4) 6 of 10 Give properties of chemostats 1) System is open 2) Fresh media in 3) Spent media and cells out 4) Continuous 5) Can be kept in 1 state 7 of 10 Change in biomass.. dx/dt = ux - Dx 8 of 10 At a steady state dx/dt=0 therefore .... D=u --> D=g 9 of 10 What is umax? When D is too high bacteria cannot stay in culture. The fastest growth rate that bacteria can grow in that media. 10 of 10
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