M:6 S:5 Biotecnology Part Two
- Created by: JessicaMPascoe
- Created on: 18-03-19 13:33
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- BiotechnologyPart Two
- Close Cultures
- When growth takes place in a vessel that is isolated from external environment
- Standard Growth Curve
- Curve
- 3. Stationary Phase
- Pop stays level
- DR = RR
- Pop stays level
- 2. Exponential Phase
- Pop ^ quickly
- Conditons = most favourable
- Pop ^ quickly
- 4. Decline Phase
- Pop falls
- 1. Lag Phase
- Pop ^ slowly
- Reproductionrate is low
- Pop ^ slowly
- 3. Stationary Phase
- N = N0 x 2^n
- Curve
- Example: Batch Fermentation
- Standard Growth Curve
- When growth takes place in a vessel that is isolated from external environment
- How to culture microorganisms
- 3. Incubate
- 2. Transfer using inoculation loop
- 4. Add nutrients
- 1. Agar Plate
- Aseptic Techniques needed
- Sterilise equipment
- Work near Bunsen Burner
- Minimise time agar plate is open
- Disinfect space
- Wear lab coat & tie long hair
- Factors affecting the growth of MOs
- Example = Temperature
- Set up 6 agar plates with the sample of bacteria & 2 that have no sample
- Place 3 in 4 degree conditions and 3 at 25 degrees
- Place one control in each of the temperatures- no colonies should grow if contamination hasn't occurred
- Place 3 in 4 degree conditions and 3 at 25 degrees
- Set up 6 agar plates with the sample of bacteria & 2 that have no sample
- QUESTIONS
- 4. Give 3 ways in which growing conditions are optimised inside a fermentation vessel
- 3. List 2 +ve and 2 -ve of the use of MOs in food production
- 5. List the stages of the standard growth curve of MOs in a closed culture
- 2. What is bioremediation?
- 6. A scientist is plating E.coli bacterial cultures on agar to investigate the effects of PH on their growth
- A. Why must he carry out the experiment using aseptic techniques?
- B. Outline 3 aseptic techniques that should be followed when culturing bacteria
- 1. What is meant by the term 'biotech ology'
- Close Cultures
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