M:6 S:5 Biotecnology Part Two

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  • BiotechnologyPart Two
    • Close Cultures
      • When growth takes place in a vessel that is isolated from external environment
        • Standard Growth Curve
          • Curve
            • 3. Stationary Phase
              • Pop stays level
                • DR = RR
            • 2. Exponential Phase
              • Pop ^ quickly
                • Conditons = most favourable
            • 4. Decline Phase
              • Pop falls
            • 1. Lag Phase
              • Pop ^  slowly
                • Reproductionrate is low
          • N = N0 x 2^n
        • Example: Batch Fermentation
    • How to culture microorganisms
      • 3. Incubate
      • 2. Transfer using inoculation loop
      • 4. Add nutrients
      • 1. Agar Plate
      • Aseptic Techniques needed
        • Sterilise equipment
        • Work near Bunsen Burner
        • Minimise time agar plate is open
        • Disinfect space
        • Wear lab coat & tie long hair
    • Factors affecting the growth of MOs
    • Example = Temperature
      • Set up 6 agar plates with the sample of bacteria & 2 that have no sample
        • Place 3 in 4 degree conditions and 3 at 25 degrees
          • Place one control in each of the temperatures- no colonies should grow if contamination hasn't occurred
    • QUESTIONS
      • 4. Give 3 ways in which growing conditions are optimised inside a fermentation vessel
      • 3.  List 2 +ve and 2 -ve of the use of MOs in food production
      • 5. List the stages of the standard growth curve of MOs in a closed culture
      • 2. What is bioremediation?
      • 6. A scientist is plating E.coli bacterial cultures on agar to investigate the effects of PH on their growth
        • A. Why must he carry out the experiment using aseptic techniques?
        • B. Outline 3 aseptic techniques that should be followed when culturing bacteria
      • 1. What is meant by the term 'biotech ology'

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