Compare and Contrast map of Electron Microscopes and Light Microscopes

Credits; http://www.ivyroses.com/Biology/Techniques/light-microscope-vs-electron-microscope.php

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  • Both use radiation to form larger and more detailed images of objects
    • Light Microscope
      • Radiation: Light
      • Used in study and research for biology and medical sciences
      • Less Expensive
      • Image  formation using light via glass lenses
      • Use the colours provided by visible light to see image
      • Image can be viewed directly by the eye
    • Electron Microscope
      • Used in study and research for biology and medical sciences
      • Uses beams of electrons rather than rays of visible light
      • Radiation: Electron Beams
      • Specimens must be carefully prepared in the correct way for both the equipment and the sample
        • Light Microscope
          • Radiation: Light
          • Less Expensive
          • Image  formation using light via glass lenses
          • Use the colours provided by visible light to see image
          • Image can be viewed directly by the eye
      • More expensive
      • Use light with a wavelength of 400-700 nm
      • Uses electron beams of approximately 1 nm wavelength
      • Image formation using electromagnets to focus beams of electrons
      • Higher magnifications
      • Produce Grey-scale images or 'false-colour' micrograph images
      • Requires use of fluorescent screen, photographic plate or electronic display to view the image because electrons cannot be seen by the human eye
      • Living specimens cannot be viewed through an electron microscope because it requires it to be vacuum in the tube

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