4.1 Cell Biology - Microscopy

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When was the first light microscope developed?
Mid 17th century
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How do light microscopes work?
Use a beam of light to form an image
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what is the highest a light microscope can magnify?
2000 times (resolving power of 200nm)
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Advantages of light microscopes?
Relatively cheap and can magnify live specimens
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When was the electron microscope invented?
1930s
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The electron microscope allowed biologists to look at the _____ structures inside cells
sub cellular
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How do electron microscopes work?
Use a beam of electrons to form an image
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What is the highest an electron microscope can magnify?
2,000,000 times (resolving power of 0.2nm)
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Compare transmission electron and scanning electron microscopes
T.E microscopes form a 2D image with very high magnifications and resolution. S.E. microscopes give dramatic 3D images but lower magnifications
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Disadvantages of electron microscopes?
Large, very expensive, and have to be kept in special temperature, pressure and humidity controlled rooms
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how do you calculate magnification?
Magnification = size of image / size of real object
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How do light microscopes work?

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Use a beam of light to form an image

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what is the highest a light microscope can magnify?

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Advantages of light microscopes?

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When was the electron microscope invented?

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