DNA fingerprinting/profiling
- Created by: Hannah Gilmore
- Created on: 15-12-12 14:00
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- DNA Fingerprinting
- 1. Isolation of DNA
- DNA is recovered from the cells + tissues of body.
- 2. Cutting and sizing of DNA
- DNA cut into fragments - either side of MRSs by a specific endonuclease
- 3. DNA Fragments are separated by Gel Electrophoresis
- Small fragments move further down the gel. Large frag. dont travel as much.
- 4. Transfer of DNA to Nylon
- Bands are heated to make them single stranded. Transferred to nylon membrane.
- Called 'Southern Blot'
- Bands are heated to make them single stranded. Transferred to nylon membrane.
- 5. Probing
- Membrane washed with Radioactive DNA Probe - binds to spec. DNA fragments.
- 6. DNA Fingerprint
- ONLY frag. detected and bound by the probe show up. The pattern of bands is called DNA Fingerprint.
- 1. Isolation of DNA
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