Testing for biochemical substances

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Test for starch
Prepare the sample you are testing, and add iodine solution. If starch is present in the sample, it will turn from a yellow-orange colour to a blue-black colour
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Test for reducing sugars
When a reducing sugar is heated with alkaline copper sulphate (Benedict’s solution) the solution will change colour from blue to an orange-red. This is called Benedict’s test
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Test for non-reducing sugars
If a substance does not react with Benedict’s solution.Boil the sample with hydrochloric acid. Cool the solution and neutralise it by adding sodium carbonate solution. Carry out the reducing sugar test again:
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Test for lipids
Mix the sample with ethanol. Pour the mixture into water contained in another test tube. If there is lipid present, a cloudy white emulsion will form near the top of the water
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Test for proteins
A protein test uses the biuret test. Biuret reagent is pale blue in colour, and is added to the sample. This results in a colour change to lilac.
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Other cards in this set

Card 2

Front

Test for reducing sugars

Back

When a reducing sugar is heated with alkaline copper sulphate (Benedict’s solution) the solution will change colour from blue to an orange-red. This is called Benedict’s test

Card 3

Front

Test for non-reducing sugars

Back

Preview of the front of card 3

Card 4

Front

Test for lipids

Back

Preview of the front of card 4

Card 5

Front

Test for proteins

Back

Preview of the front of card 5

Comments

Bethany Cunningham

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You need to say "potassium in iodine solution" to ensure the mark for test in starch. 

Also in the reducing sugars, if you are doing OCR, you need to know how to test the concentration of glucose. I have a resource for that!

Great resource though :)

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