Measurement of drug concentration

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What different methods of measuring drug concentration?
Physicochemical - mass spec, IR/UV spectroscopy. Bioassay-2x2 bioassay. Immunoassays-interaction of antigen/ab. radioimmunoassay, enzyme-linked immunoabsorbent assay
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Describe Bioassay.
measuring drug conc by measuring response in relation to a standard preparation. log M=EC50 test/EC50 standard. if M was 10 you would need 10 more test drug, so much have 10x less active substance.
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Describe an enzyme-linked immunoabsorbent assay ELISA-antigen detection
To detect antigen-ab coated filter, wash, add soltuion, wash, add second ab with enzyme that is against antigen. incubuate with enzyme substrate. if colour forms then hormone present
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ELISA-non competitive to detect antibody
antigen coated, incubate with sample, wash, incubate with enzyme linked to 2ab (against 1 ab) if colour then antibody present. Same for competitive ELISA but if colour produce no antibody present.
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Describe a radioimmunoassay.
The more hormone that binds the less radioactivity is detected. Use standard curves.. Incubate with unknown hormone, incubate with radioactive hormone, measure radioactivity. hormone will push radiolabelled hormone out.
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Other cards in this set

Card 2

Front

Describe Bioassay.

Back

measuring drug conc by measuring response in relation to a standard preparation. log M=EC50 test/EC50 standard. if M was 10 you would need 10 more test drug, so much have 10x less active substance.

Card 3

Front

Describe an enzyme-linked immunoabsorbent assay ELISA-antigen detection

Back

Preview of the front of card 3

Card 4

Front

ELISA-non competitive to detect antibody

Back

Preview of the front of card 4

Card 5

Front

Describe a radioimmunoassay.

Back

Preview of the front of card 5

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