Mass spectrometry 0.0 / 5 ? PharmacyAnalytical techniquesUniversityNone Created by: SamDaviesCreated on: 07-04-19 20:01 83526147 Across 1. This type of mass spec involves fragmenting the fragments to identify any peaks that may come from impurities. (6) 4. Ions pass through an oscillating electrostatic field generated by charged rods - the path of the ions is altered by the field depending on the m/z ratio. By altering the field strength, the spectrometer can detect different m/z ratios (10) 5. These analysers accelerate ions using an electrostatic field of known strength. Smaller and more highly charged ions accelerate faster. m/z is determined by the time taken to reach the detector (4, 2, 6) 7. A vaporised sample is exposed to chemical ions (e.g. CH3+, CH4+, CH5+) formed by EI. Can result in protonation, abstraction, adduct formation or charge exchange (8, 10) Down 2. Machine which separates the ions (8) 3. This type of mass spec can be used to determine the mass of an ion up to 4 decimal places. This means the ion can be linked to a single molecular formula (not useful for isomers) (4, 10) 6. A magnetic or static field electric field is used to deflect ions. Smaller and more highly charged ions are deflected more (m/z) (6) 8. A vaporised sample is exposed to a beam of electrons, resulting in abstraction or addition of electrons. The radical ions are very reactive and have a low stability, leading to a high degree of fragmentation (hard ionisation) (8, 10)
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