Histology

?
What are the 8 steps to prepare a slide?
1. tissue collection
2. fixation and trimming
3. dehydration
4. paraffin embedding
5. section with a microtome
6. straighten in a water bath
7. transfer to a slide
8. stain and coverslip
1 of 18
Haematoxylin
- colour?
- what does it stain?
blue
stains genetic material
2 of 18
Eosin
- colour?
- what does it stain?
Pink
stains proteins
3 of 18
Types of single layer epithelia?
(4)
simple squamous
simple cuboidal
simple columnar
simple pseudostratified columnar
4 of 18
Types of stratified epithelia?
(4)
stratified squamous
stratified cuboidal
stratified columnar
stratified transitional
5 of 18
2 Types of epithelial glands?
endocrine and exocrine
6 of 18
Endocrine glands
no duct system
close to blood vessels
secrete hormones
7 of 18
Exocrine glands
can be simple or branched
tubular or acinar
8 of 18
Serous secretion
Clear and watery
9 of 18
Mucous secretion
thick and viscous
10 of 18
Mixed secretion
a mixture between serous and mucous
11 of 18
Merocrine secretion
exocytosis
cell membrane remains intact
12 of 18
Apocrine secretion
decapitation of cell
13 of 18
Holocrine secretion
cell ruptures and dies releasing what is inside
14 of 18
Cell junctions
(3)
adherent
tight/occluding
gap
15 of 18
Adherent cell junctions
link cells together
cells that are linked to other cells by: desmosomes
cells that are linked to the matrix by: hemidesmosomes
16 of 18
Tight/occluding cell junctions
controls what enters and leaves the cell
Transcellular - movement through the cell
Paracellular - movement between the cells
17 of 18
Gap cell junctions
help cells to communicate
18 of 18

Other cards in this set

Card 2

Front

Haematoxylin
- colour?
- what does it stain?

Back

blue
stains genetic material

Card 3

Front

Eosin
- colour?
- what does it stain?

Back

Preview of the front of card 3

Card 4

Front

Types of single layer epithelia?
(4)

Back

Preview of the front of card 4

Card 5

Front

Types of stratified epithelia?
(4)

Back

Preview of the front of card 5
View more cards

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