2x Triose phosphate --> 2x phosphoglycerate. Oxidation occurs, involving removing 2 hydrogen atoms from each triose phosphate molecule. ATP formed.
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6th step
2x phosphoglycerate --> 2x pyruvate. ATP formed for each reaction. Hydrogen atom pairs accepted by NAD.
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Gross and net production of ATP
Gross production = 4, net production = 2.
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Reduced NAD produced?
2NADH2 produced as NAD has gained hydrogen.
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Where does the link reaction take place?
Inside the fluid matrix of the mitochondrion
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What happens in the link reaction?
Pyruvic acid is converted to Acetyl CoA. Coenzyme A is recycled.
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What is produced in the link reaction?
2 hydrogen atoms, carbon dioxide and NADH2.
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Where does the Krebs cycle take place?
In the fluid matrix of the mitochondrion.
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Stage 1 of Krebs Cycle
4C Oxaloacetic acid --> 6C Citric acid. 2C group of Acetyl CoA combines with 4C Oxaloacetic acid to form 6C Citric acid.
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2nd stage of Krebs Cycle
6C Citric acid --> 5C Keto Glutaric acid. 6C oxidised to 5C. Decarboxylation + dehydrogenation. Produces CO2, hydrogen and NADH2.
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3rd stage of Krebs Cycle
5C Keto Glutaric acid --> 4C Succinic acid. 5C oxidised to 4C. Generates CO2 and NADH2. Decarboxylation + dehydrogenation. 2 hydrogens produced, ATP produced by substrate-level phosphorylation.
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Stage 4 of Krebs cycle
4C Succinic acid --> 4C Malic acid. Oxidised. Hydrogen carrier = FAD. 2 hydrogens produced and FADH2.
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