BMS1031 Bacterial Genetics Example Q's

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1. cDNA cloning:

  • does not require ligation
  • requires genomic DNA
  • requires the enzyme reverse transcriptase
  • requires bacteriophages
  • does not require thought
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Other questions in this quiz

2. Single base substitution in the codon UUA (leu) to form UCA (ser) represents a...

  • missense mutation
  • silent mutation
  • nonsense mutation
  • transversion mutation
  • frameshift mutation

3. How many different transcription factors are bound to the E.coli lac operon promoter region when both lactose and glucose are present in the growth medium?

  • 4
  • 3
  • none
  • 2
  • 1

4. To express a eukaryotic gene in a bacterium:

  • B. firstly cDNA has to be synthesised from the mature mRNA before cloning the gene
  • A and B
  • A. a bacterial expression vector must be used
  • C. the genomic DNA first needs to be cloned from the eukaryotic genome
  • all of the above

5. When E.coli is grown on a mixture of lactose and glucose?

  • lactose is never used
  • lactose represses glycolysis
  • lactose is used first
  • glucose is used first
  • lactose and glucose are used at the same time

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