Why are microbes always treated as 'potentially deadly'?
Even if the bottle containing microbes is labelled, there may be other harmful microbes growing in the bottle, due to contamination or mutated bacteria.
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Why is the neck of the bottle containing the bacteria/agar flamed?
To sterilise it and kill any bacteria that may have contaminated the neck of the bottle, e.g. from dust.
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How are the forceps (used to transfer antibiotic discs) sterilised?
Left in ethanol and flamed before use.
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Why is the lid of the petri dish not sealed all the way around?
To ensure that oxygen is available to the bacteria, preventing potentially harmful anaerobic bacteria from growing.
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At what temperature and for how long should the bacteria be incubated in a school lab?
20 degrees celsius for around 48 hours.
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Why is the bunsen burner left on throughout the experiment?
To generate an upward current and therefore keep the area of the bench next to it sterile.
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How is the most effective antibiotic determined?
By measuring the areas of the zones of inhibition around each test disc and comparing them.
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What should be labelled on the base of the agar plate?
Date, species of bacteria, initials
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Other cards in this set
Card 2
Front
Why is the neck of the bottle containing the bacteria/agar flamed?
Back
To sterilise it and kill any bacteria that may have contaminated the neck of the bottle, e.g. from dust.
Card 3
Front
How are the forceps (used to transfer antibiotic discs) sterilised?
Back
Card 4
Front
Why is the lid of the petri dish not sealed all the way around?
Back
Card 5
Front
At what temperature and for how long should the bacteria be incubated in a school lab?
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