- Add a solution of iodine (in potassium iodide).
- The iodine solution changes from yellow to blue/black.
- Heat with Benedict's solution (alkaline copper sulfate).
- The colour changes from blue to orange/red.
- A precipitate is formed.
- The non-reducing sugar will not react with the Benedict's solution.
- Boil the sample with hydrochloric acid.
- This hydrolyses any sucrose present, splitting sucrose molecules to give glucose and fructose monosaccharides.