Required practical: Investigate the effect of pH on the rate of reaction of amylase enzyme

Investigate the effect of pH on the rate of reaction of amylase enzyme

Method 

1. Put a test tube containing starch soultion and a test tube containing amylase into a water bath at 37 degrees celusis

2. After 5 minutes add the amylase solution to the starch. 

3. Every 30 seconds take a drop from the mixture and test it for starch using iodine solution 

4. Record how long it takes for the starch to be completely digested

5. Repeat the experiment at different pH values using different buffer solutions 

Variables

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