Microscopy
- Created by: raffia99
- Created on: 01-10-16 21:50
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Light Microscope
- Large cellular stuructures can be seen (nucleus, cell wall, chloroplasts, vacuole, mitochondria)
- Cheaper to buy/operate
- Small, portable
- Simple to use, easy sample preperation
- Material rarely distorted by sample preperation
- Natural colour of sample maintained
- Magnifies to x2000
- Only cell exterior can be seen
- Light produced from internal/ external light source
- Passees through diaphragm (hole which controls ammount of light which reaces specimen)
- light passes through condesnser
- sondenser focuses light on specimen
- slide held on stage at 90° to path of light
- light travels through specimen
- objective lens magnifies image of specimen
- light travels through barrel of microscope
Electron Microscopes
- Magnifies over x500 000
- Resolution is less than 1nm
- Expensive to buy, produce…
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