Culturing Microorganisms

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HOW YOU CAN GROW MICROORGANISMS AND TEST HOW EFFECTIVE DIFFERENT ANTIBIOTICS ARE AT KILLIN THEM.

YOU CAN GROW BACTERIA IN THE LAB

  • BACTERIA (and other microorganisms) are grown(cultured) in a culture medium which contains the carbohydrate, minerals, protiens and vitimins they need to grow.
  • The culture medium used can be a nutrient broth solution or solid agar jelly.
  • BACTERIA grown on agar plates will form visible colonies on surface of the jelly, or will spread out to give an even covering of bacteria.

To makean agar plate, hot agar jelly is poured into a peti dish

When jelly cools and sets, an inculating loop(wire loop)can be used to transfer microorgansims to the culture medium.Altenatively, a sterile dropping pipette and spreader can be used to get and even covering of bacteria.Microorganisms then multiply.

  • In school lab, cultures of microorganisms are not kept above 25*C because harmful pathogens are more likely to grow in higher temperatures.
  • Industrial conditions, cultures are incubated at igher temperatures so they can grow faster.

INVESTIGATING THE EFFEECT OF ANTIBIOTICS ON BACTERIAL GROWTH

1) Place paper discs soaked in different types(or concentrations)of antibiotics on an agar plate and that has an even covering of bacteria.Lave space between discs.

2)The antibioics should diffuse into agar jelly.

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