Meselson and Stahl used heavy and light strands of DNA. They did this by using DNA from bacteria that had been grown in a medium containing only the heavy isotope of nitrogen, 15N. All the nucleotides in the bacteria at the start of their experiment contained heavy nitrogen, and this made the DNA denser than normal.
Meselson and Stahl then moved the bacteria into a medium containing only normal 14N. This meant all the new nucleotides incorporated into the replicated DNA were 'light' but the original DNA nucleotides were heavy. They allowed the bacteria to divide and their DNA to replicate once. They then extracted and centrifuged the DNA.
If a test tube containing DNA dissolved in a special density-gradient solution is centrifuged the heavy DNA (containing 15N) sinks to the bottom. Light DNA (containing normal 14N) collects a band near the top, and DNA of medium density it in the middle. Medium density DNA must contain some heavy and some light nucleotides.
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