Castrated rodents show little or no aggressive behaviour. However, if testosterone is replaced, they will show typical aggressive behaviour.
Injecting neonatal female rodents with testosterone made them much more aggressive when given testosterone as adults compared to female controls. This supports the idea that the sensitisation of neural cicuitry after birth is an important factor in the effect of testosterone release.
However, experiments on animals lacks generalisability as brain areas said to be affected by testosterone serve different functions accross species.
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