DNA SEQUENCING- The DNA of organisms can be compared by looking at their DNA base sequences. Closely related species will have a higher percentage of of similarity in the DNA base sequence.
DNA HYBRIDISATION- DNA from two different species are separated into strands and mixed together. Where the DNA base sequence is the same (complimentary), hydrogen bonds form between between bases by specific bas pairing. The more that hybridise the more alike the DNA is. Then DNA is heated to separate it out into strands again, the higher the temperature, the more hydrogen bonds formed (so more energy needed to break them).
Comparing amino acid sequence: Proteins are made from amino acids, the sequence of amino acids is coded for by the DNA base sequence. So similar organisms with similar DNA will have mroe similar amino acid sequences.
Immunilogical comparisons: Antibodies will bind to similar shaped proteins, so any protein that is a simialr shape to the antibodies of a specific species then it will bind.
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