root tip squash

rooot tip squash method

ut off 1-2 cm of the root tips. Put in a small volume of ethanoic acid on a watchglass (or other shallow dish) for 10 minutes. Meanwhile, heat 10-25 cm3 of 1 M hydrochloric acid to 60 °C in a water bath. Wash the root tips in cold water for 4-5 minutes and dry on filter paper. Use a mounted needle to transfer the root tips to the hot hydrochloric acid (see b) and leave for 5 minutes. Wash the root tips again in cold water for 4-5 minutes and dry on filter paper. Use the mounted needle to remove two root tips onto a clean microscope slide. Add a small drop of stain and leave for 2 minutes Break up the tissue with a mounted needle. Cover with a coverslip and squash Calculate the mitotic index. Compare the mitotic index of root tips from alliums that have been sprouting for 1, 2, 5 and 10 days. If available, compare the allium meristem tissue with that from other species.

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