PHOTOSYNTHESIS

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  • PHOTOSYNTHESIS
    • LIMITING FACTORS
      • light intensity
      • carbon dioxide (0.04%)
      • temperature
    • ADVANTAGES OF PLANT STRUCTURE
      • leaf
        • LARGE surface area  short diffusion distance
      • palisade cell
        • closely packed together
      • chloroplast
        • many grana & thylakoids are parallel
      • chloroplast
        • lipid soluble tail
      • many photosynthetic pigments
        • large range of light intensities & wavelengths
        • chlorophyll a & b
        • carotene (high levels of light)
      • antenna complex
        • low light intensities
        • absorb & reflect light to reaction center (chlorophyll a)
      • absorption spectra
        • absorption of light
      • action spectra
        • rate of photosynthesis & wavelength of light
    • CALVIN EXPERIMENT
      • lollipop flask
        • LARGE surface area
      • screening glass
        • stop overheating & death of enzymes
      • nutrients reservoir
        • amino acids & proteins for growth
      • sodium hydrogen carbonate
        • carbon dioxide
      • radioactive carbon-14
        • radioactive marker
      • hot methanol
        • "suspended animation"
      • Chromatography (TLC)
        • black radioactive spots
        • pigment soluble in solvent
          • more soluble = carried further
        • compare with known Rf values
        • 2-way chromatography = rotate 90'
    • MEASURING RATE OF PHOTOSYNTHESIS
      • Potometer Experiment
      • = (pie r2 x length or distance bubble moved) DIVIDED BY time
    • LIGHT DEPENDENT REACTION
      • occurs on the thylakoid membrane
      • The Light Dependent Reaction
      • products
        • ATP
        • oxygen
        • NADPH
    • LIGHT-INDEPENDENT REACTION
      • occurs in the stroma
      • products from light dependent reactions
        • NADPH
        • ATP
      • The Light-Independent Reaction

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