light vs electron microscope
- Created by: sophiagbrown
- Created on: 01-10-14 11:58
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- light vs electron microscope
- Electron Microscope
- use beams of electron with a very short wavelength meaning the resolution is higher
- sample needs to be 'ultra thin' so the electrons can pass through
- specimen needs to be dead as it needs to be stained by heavy metals and sliced very thinly
- scanning electron microscope- specimen prepared in the same way
- however electrons are reflected from the surface producing a 3d image
- transmission electron microscope- specimen must be covered in dark resin to produce a solid block
- electrons are focused by electromagnets onto a fluorecent screen or photographic film
- light microscope
- can only distinguish between 2 objects that are only 0.2 nano meters apart
- limited magnification of 1500x
- can view living specimens
- colors and dyes can be used to make structures more visible
- image is in color
- Electron Microscope
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