Lederberg and Tatum
- Created by: Jamie Thomas
- Created on: 16-03-14 10:59
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- Lederberg and Tatum- E.coli
- Lederberg observed that bacteria produced clones of itself down the generations.
- He teamed up with Tatum to test his hypothesis that bacteria passed on bacteria across generations, this was a different means of variation other than mutation
- They used 2 different strains of E.coli crown in mediums containing nutrients. Neither strain could grow with the basic nutrients that were in the medium.
- Strain 1could not synthesis the amino acid Methionine and the vitamin biotin, so could not grow.
- Strain 2 could not synthesis Threonine and Leucine so could not grow.
- They mixed the 2 strains then grew them on a medium containing all missing supplements.
- The bacteria where then removed by centrifugation and washed .
- They were then transferred into 2 mediums with either all the supplements or minimal supplements.
- Though the bateria did not grow 1 in 10 million did divide and develop into a colony.
- This was used as proof of conjugation.
- Though the bateria did not grow 1 in 10 million did divide and develop into a colony.
- They were then transferred into 2 mediums with either all the supplements or minimal supplements.
- The bacteria where then removed by centrifugation and washed .
- They mixed the 2 strains then grew them on a medium containing all missing supplements.
- Strain 2 could not synthesis Threonine and Leucine so could not grow.
- Strain 1could not synthesis the amino acid Methionine and the vitamin biotin, so could not grow.
- They used 2 different strains of E.coli crown in mediums containing nutrients. Neither strain could grow with the basic nutrients that were in the medium.
- He teamed up with Tatum to test his hypothesis that bacteria passed on bacteria across generations, this was a different means of variation other than mutation
- Lederberg observed that bacteria produced clones of itself down the generations.
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